From 2007.igem.org

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Revision as of 22:38, 8 June 2007

Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the HPI, katG open reading frame from Salmonella typhimurium LT2

PCR VU03F.katG /VU06R.katG on HPI, katG	(1316 bp, gp = A)
PCR VU05F.katG/VU04R.katG on HPI, katG		(924 bp, gp = B)
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PCR VU03F/VU04R on A+B				(2212 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (Clone # 5)		(BglII/XhoI, 2063+910, L)
Product is pBca9145-VU2 
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VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc VU04R.katG (External oligo) gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc