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Revision as of 16:47, 21 October 2007 (view source) Nakatani (Talk | contribs) ← Older edit		Revision as of 17:26, 21 October 2007 (view source) Nakatani (Talk | contribs) Newer edit →
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	===2-0.Wet : [[Tokyo/Hybrid promoter|Hybrid promoter]]===		===2-0.Wet : [[Tokyo/Hybrid promoter|Hybrid promoter]]===
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	[[Image:Hybrid.JPG|200px|]]		[[Image:Hybrid.JPG|200px|]]
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	===2-1.Dry : [[Tokyo/Formulation |Formulation]]===		===2-1.Dry : [[Tokyo/Formulation |Formulation]]===
	We writed the Formulation		We writed the Formulation

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Revision as of 17:26, 21 October 2007

砂場2

1,Project Concept & Model We made bacterial society that follows Pareto's principle,for example ,Ant society Requirements Our requirements for construction this model are two things,bistability and coexistence by cell-cell communication Genetic circuit We constructed the genetic circuit to fill its requirements. Therefore we made new parts Our construction parts are Here!!!

2,Works Approach:We alternated approach of Wet and Dry. 2-0.Wet : Hybrid promoter

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2-1.Dry : Formulation

We writed the Formulation

2-2.Wet : Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

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2-3.Dry : Simulation

wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team

Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba