Tokyo/sunaba2
From 2007.igem.org
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====We need to develop the promoter that can receive two input,AHL & reperssor.We tryed to create and [[Tokyo/Hybrid promoter|successed]]!! ==== | ====We need to develop the promoter that can receive two input,AHL & reperssor.We tryed to create and [[Tokyo/Hybrid promoter|successed]]!! ==== | ||
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[[Image:Hybrid.JPG|200px|]] | [[Image:Hybrid.JPG|200px|]] | ||
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===2-1.Dry : [[Tokyo/Formulation |Formulation]]=== | ===2-1.Dry : [[Tokyo/Formulation |Formulation]]=== | ||
We writed the Formulation | We writed the Formulation | ||
Revision as of 17:32, 21 October 2007
砂場2
1,ProjectConcept & ModelWe made bacterial society that follows Pareto's principle,for example ,Ant society
RequirementsOur requirements for construction this model are two things,bistability and coexistence by cell-cell communication
Genetic circuitWe constructed the genetic circuit to fill its requirements. Therefore we made new parts
Our construction parts are Here!!! |
2,WorksApproach:We alternated approach of Wet and Dry.2-0.Wet : Hybrid promoter
2-1.Dry : FormulationWe writed the Formulation 2-2.Wet : AssayWe decided the parameter of hill coefficients by the assay of AHL & IPTG
2-3.Dry : Simulationwet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
2-4.Wet : AssaySimulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
2-5.Future works3,About our team
Advisors: Last year's member
Instructors
Links sunaba |
