Bologna University/M9 medium
From 2007.igem.org
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::3.If desired aseptically add 0.1mL of 1M sterile calcium chlorid to the M9 minimal medium. The M9 minimal medium may also be supplemted with the appropriate amino acids. | ::3.If desired aseptically add 0.1mL of 1M sterile calcium chlorid to the M9 minimal medium. The M9 minimal medium may also be supplemted with the appropriate amino acids. | ||
| - | [[Bologna University/Protocols/Mediums and | + | [[Bologna University/Protocols/Mediums and Buffer | Back]] |
Revision as of 09:02, 22 October 2007
Preparation instructions:
- 1.Dissolve 56.4g in 1L of distilled water.
- 2.Autoclave for 15min at 121°C.
This convenient 5x concentrate can be stored and diluited as needed to prepare 5L of 1x M9 minimal salts. For M9 minimal medium:
- 1.Aseptically dilute 200mL of M9 minimal salts, 5x concentrate with 800mL of sterile water, if necessary, cool to 45-50°C.
- 2.Aseptically add 20mL of sterile 1M glucose and 2mL of sterile 1M magnesium solfate to prepare 1L of M9 minimal medium.
- 3.If desired aseptically add 0.1mL of 1M sterile calcium chlorid to the M9 minimal medium. The M9 minimal medium may also be supplemted with the appropriate amino acids.
