Freiburg07/primers
From 2007.igem.org
(→Primers for calmodulin-construct with different linkerlengths) |
(→Primers for calmodulin-construct with different linkerlengths) |
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forward primers:<br> | forward primers:<br> | ||
| - | pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'<br> | + | pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3' <br> |
| - | pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3'<br> | + | pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3' <br> |
| - | pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3'<br> | + | pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3' <br> |
| - | pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3'<br> | + | pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3' <br> |
| - | pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3'<br> | + | pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3' <br> |
| + | |||
| + | reverse primers:<br> | ||
| + | |||
| + | pr-calmo-SpeI-linker2: 5' AACGATCACT AGTACCTCCC TTTGCTGTCA TCATTTGTAC A 3' <br> | ||
| + | pr-calmo-SpeI-linker3: 5' AACGATCACT AGTACCGCCA CCGCCCTTTG CTGTCATCAT TTGTACA 3' <br> | ||
| + | pr-calmo-SpeI-linker4: 5' AACGATCACT AGTGCCACCG CCTCCCTTTG CTGTCATCAT TTGTACA 3' <br> | ||
| + | pr-calmo-SpeI-linker6: 5' AACGATCACT AGTACCGCCA CCAGAGCCAC CCTTTGCTGT CATCATTTGT ACA 3' <br> | ||
| + | pr-calmo-SpeI-linker9: 5' AACGATCACT AGTACCGCCA CCGCCAGAAC CGCCACCGCC CTTTGCTGTC ATCATTTGTA CA 3' <br> | ||
Revision as of 14:14, 22 October 2007
Primers for specific ends of the dhfr1-fragment
pr-Kpn1-dhfr1: 5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'
pf-Sph-h-dhfr1: 5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'
Primers for calmodulin-construct with different linkerlengths
forward primers:
pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'
pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3'
pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3'
pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3'
pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3'
reverse primers:
pr-calmo-SpeI-linker2: 5' AACGATCACT AGTACCTCCC TTTGCTGTCA TCATTTGTAC A 3'
pr-calmo-SpeI-linker3: 5' AACGATCACT AGTACCGCCA CCGCCCTTTG CTGTCATCAT TTGTACA 3'
pr-calmo-SpeI-linker4: 5' AACGATCACT AGTGCCACCG CCTCCCTTTG CTGTCATCAT TTGTACA 3'
pr-calmo-SpeI-linker6: 5' AACGATCACT AGTACCGCCA CCAGAGCCAC CCTTTGCTGT CATCATTTGT ACA 3'
pr-calmo-SpeI-linker9: 5' AACGATCACT AGTACCGCCA CCGCCAGAAC CGCCACCGCC CTTTGCTGTC ATCATTTGTA CA 3'
