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Line 3:		Line 3:
	::-I763019;		::-I763019;
	::-pTet-LacI-T-Plambda-RFP.		::-pTet-LacI-T-Plambda-RFP.
-	*Run on electrophoresis gel and I763019 band extraction.	+
Line 13:		Line 13:
	::-I763015;		::-I763015;
	::-I763004.		::-I763004.
-		+	*Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
Line 22:		Line 22:
	::-I763004.		::-I763004.
	*We inoculate:		*We inoculate:
-	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;	+	::-Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;
	::-pTet-LacI-T-pLambda-rfp (high copy).		::-pTet-LacI-T-pLambda-rfp (high copy).
-	éDigestion of	+	*Control Digestion for results impagination (X/P):
		+	::- I763020;
		+	::- I763004;
		+	::- I763011;
		+	::- I763007 (on high copy plasmid);
		+	::- I763007 (on low copy plasmid);
		+	::- I763033;
		+	::- I763015;
		+	::- I763012;
		+	::- I763005;
		+	::- I763031.
		+

---

Revision as of 08:35, 23 October 2007

10/15/07

• O/N ligations for:

-I763019;

-pTet-LacI-T-Plambda-RFP.

10/16/07

• Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
• We inoculate:

-I763005;

-I763015;

-I763004.

• Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;

-pTet-LacI-T-pLambda-rfp (high copy).

• Control Digestion for results impagination (X/P):

- I763020;

- I763004;

- I763011;

- I763007 (on high copy plasmid);

- I763007 (on low copy plasmid);

- I763033;

- I763015;

- I763012;

- I763005;

- I763031.

10/18/07

10/19/07

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