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Line 2:		Line 2:
	*O/N ligations for:		*O/N ligations for:
	::-I763019;		::-I763019;
-	::-pTet-LacI-T-Plambda-RFP.	+	::-I763039.
Line 8:		Line 8:
	::'''10/16/07'''		::'''10/16/07'''
-	*Transformation of I763019 and pTet-LacI-T-Plambda-RFP.	+	*Transformation of I763019 and I763039.
	*We inoculate:		*We inoculate:
	::-I763005;		::-I763005;
Line 40:		Line 40:
	::'''10/18/07'''		::'''10/18/07'''
	*Miniprep:		*Miniprep:
-	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;	+	::-I763029;
-	::-pTet-LacI-T-pLambda-rfp (high copy).	+	::-I763039(high copy).
	*Digestion for:		*Digestion for:
-	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)	+	::-I763029 (E/P)
	*Band extraction of:		*Band extraction of:
-	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)	+	::-I763029 (E/P)
	*Ligation for:		*Ligation for:
	::-pSB4A3 (E/P)		::-pSB4A3 (E/P)
-	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)	+	::-I763029 (E/P)
	*Ligation for:		*Ligation for:
-	::-pLac-GFP(X/P)	+	::-I763004 (X/P)
-	- pTetR-LacI (S/P)	+	::-I763026 (S/P)
	*Control Digestion for results impagination:		*Control Digestion for results impagination:
Line 73:		Line 73:
	::'''10/19/07'''		::'''10/19/07'''
	*Transformation:		*Transformation:
-	::-pLac-GFP-pTet-LacI (on high copy plasmid);::	+	::-I763040(on high copy plasmid);::
-	::-pTet-LacI-T-Plambda-RFP;	+	::-I763039;
-	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).	+	::-I763029 on pSB4A3 (low copy plasmid).

---

Revision as of 08:58, 23 October 2007

10/15/07

• O/N ligations for:

-I763019;

-I763039.

10/16/07

• Transformation of I763019 and I763039.
• We inoculate:

-I763005;

-I763015;

-I763004.

• Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;

-pTet-LacI-T-pLambda-rfp (high copy).

• Control Digestion for results impagination (X/P):

- I763020;

- I763004;

- I763011;

- I763007 (on high copy plasmid);

- I763007 (on low copy plasmid);

- I763033;

- I763015;

- I763012;

- I763005;

- I763031.

10/18/07

• Miniprep:

-I763029;

-I763039(high copy).

• Digestion for:

-I763029 (E/P)

• Band extraction of:

-I763029 (E/P)

• Ligation for:

-pSB4A3 (E/P)

-I763029 (E/P)

• Ligation for:

-I763004 (X/P)

-I763026 (S/P)

• Control Digestion for results impagination:

- I763016 (low copy plasmid);

- I763016 (high copy plasmid);

- I763036;

- I763019;

- I763032;

- I763025 (low copy plasmid);

- I763025 (high copy plasmid);

- I763026;

- I763035.

10/19/07

• Transformation:

-I763040(on high copy plasmid);::

-I763039;

-I763029 on pSB4A3 (low copy plasmid).

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