Freiburg07/plasmids
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'''Sequence of Calmodulin''': [[Freiburg07/Cam_yc6E_2|Cam_yc6E_2 ]]<BR> | '''Sequence of Calmodulin''': [[Freiburg07/Cam_yc6E_2|Cam_yc6E_2 ]]<BR> | ||
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== New plasmids == | == New plasmids == | ||
Revision as of 12:49, 23 October 2007
Origin plasmids
Ca2+ sensor: ß-lactamase
Plasmid containing the lactamase part 1 fragment: pAR310d-blac1-winzipA2
Plasmid containing the lactamase part 1 fragment with periplasmatic signal sequence: pAR310dp-blac1-winzipA2
Plasmid containing the lactamase part 2 fragment: pAR300d-winzipB1-blac2
Plasmid containing the lactamase part 2 fragment with periplasmatic signal sequence: pAR300dp-winzipB1-blac2
Ca2+ sensor: DHFR
Plasmid containing the dhfr1 fragment: pAR200d-GAS41reg1-dhfr1
Plasmid containing the dhfr2 fragment: pAR300d-AF10winDelV41D-dhfr2A
Light sensor: Phytochrom A with CFP and YFP
Plasmid containing PhyA1: D153ah-phyA_1-406
Plasmid containing FHY1: pGADT7ah-FHY1
Plasmid containing CFP: pAR200-cJunCFP
Plasmid containing YFP: pAR200-cFos-YFP
Plasmid containing YFP: pAR200-YFP-Dumy
Sequence of Calmodulin: Cam_yc6E_2
New plasmids
pAR200-Fhy1Big-YFP
pAR200-Fhy1Small-YFP
pAR200-PhyA-His-CFP
pAR200-YFP-Fhy1Big
pAR200-YFP-Fhy1Small
Plasmid wih both lactamase fragments: pFR320d-blac1-winzipB1-blac2
Plasmid wih both lactamase fragments and periplamatic signal sequence: pFR320p_b1_WinzipB1_b2
Plasmids with calmodulin between the lactamase fragments:
2 amino acids for linker: pFR320p_b1_2calmo2_b2
4 amino acids for linker: pFR320p_b1_4calmo4_b2
6 amino acids for linker: pFR320p_b1_6calmo6_b2
Plasmids with calmodulin between the dhfr fragments:
2 amino acids for linker: dFR320d-dhfr1-2calmo2-dhfr2A
4 amino acids for linker: dFR320d-dhfr1-4calmo4-dhfr2A
6 amino acids for linker: dFR320d-dhfr1-6calmo6-dhfr2A
