Tokyo/Works/Hybrid promoter
From 2007.igem.org
(→Design) |
|||
| Line 11: | Line 11: | ||
<br>-Lutz, R., and H. Bujard. 1997. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-1-I-2 regulatory elements. Nucleic Acids Res. 25:1203–1210. | <br>-Lutz, R., and H. Bujard. 1997. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-1-I-2 regulatory elements. Nucleic Acids Res. 25:1203–1210. | ||
| - | == | + | ==Experiments== |
| + | The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though [[Tokyo/Works/Assay |Assay1]] and [[Tokyo/Works/Assay |Assay2]]. | ||
Revision as of 16:12, 23 October 2007
Works top 0. Hybrid promoter 1. Formulation 2. Assay1 3. Simulation 4. Assay2 5. Future works
Purpose
To realize our model, expression of one part of the genes should be regulated by two factors. Therefore, the promoter upstream of the idlers genetic part was designed to sense TWO INPUTS, activated by AHL and repressed by LacI.
Design
Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER.
References
-Biobrick R0062
-Lutz, R., and H. Bujard. 1997. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-1-I-2 regulatory elements. Nucleic Acids Res. 25:1203–1210.
Experiments
The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though Assay1 and Assay2.
