Tokyo/Works/Hybrid promoter
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<br>[[Tokyo/Works|Works top]] 0. [[Tokyo/Works/Hybrid promoter|Hybrid promoter]] 1. [[Tokyo/Works/Formulation |Formulation]] 2. [[Tokyo/Works/Assay |Assay1]] 3. [[Tokyo/Works/Simulation |Simulation]] 4. [[Tokyo/Works/Assay2 |Assay2]] 5. [[Tokyo/Works/Future works |Future works]] | <br>[[Tokyo/Works|Works top]] 0. [[Tokyo/Works/Hybrid promoter|Hybrid promoter]] 1. [[Tokyo/Works/Formulation |Formulation]] 2. [[Tokyo/Works/Assay |Assay1]] 3. [[Tokyo/Works/Simulation |Simulation]] 4. [[Tokyo/Works/Assay2 |Assay2]] 5. [[Tokyo/Works/Future works |Future works]] | ||
==Purpose== | ==Purpose== | ||
| + | [[Image:Hybrid.JPG|thumb|150px| '''Fig.1: '''Genetic Circuit|right]] | ||
To realize our model, expression of one part of the genes should be regulated by two factors. Therefore, the promoter upstream of the idlers genetic part was designed to '''sense TWO INPUTS, activated by AHL and repressed by LacI'''. | To realize our model, expression of one part of the genes should be regulated by two factors. Therefore, the promoter upstream of the idlers genetic part was designed to '''sense TWO INPUTS, activated by AHL and repressed by LacI'''. | ||
| + | <br> | ||
==Design== | ==Design== | ||
Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a '''"HYBRID"''' of the two, we call it '''HYBRID PROMOTER'''. | Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a '''"HYBRID"''' of the two, we call it '''HYBRID PROMOTER'''. | ||
| - | + | [[Image:Hybridpromoter.JPG|thumb|350px| '''Fig.1: '''Genetic Circuit|right]] | |
<br>'''References''' | <br>'''References''' | ||
<br>-Biobrick R0062 | <br>-Biobrick R0062 | ||
Revision as of 17:19, 23 October 2007
Works top 0. Hybrid promoter 1. Formulation 2. Assay1 3. Simulation 4. Assay2 5. Future works
Purpose
To realize our model, expression of one part of the genes should be regulated by two factors. Therefore, the promoter upstream of the idlers genetic part was designed to sense TWO INPUTS, activated by AHL and repressed by LacI.
Design
Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER.
References
-Biobrick R0062
-Lutz, R., and H. Bujard. 1997. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-1-I-2 regulatory elements. Nucleic Acids Res. 25:1203–1210.
Experiments
The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though Assay1 and Assay2. The results are briefly shown in Fig.
