Tokyo/Requirements
From 2007.igem.org
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The most important and underlying point of our model is that the system is stable against environmental changes when two or more types of individuals coexist, called "coexistence stability." Our project have aimed at this "coexistence stability," not dynamic equilibrium such as chemical ones | The most important and underlying point of our model is that the system is stable against environmental changes when two or more types of individuals coexist, called "coexistence stability." Our project have aimed at this "coexistence stability," not dynamic equilibrium such as chemical ones | ||
| - | 共存安定をなりたたせるために必要な要素は以下の３つである | + | <!--共存安定をなりたたせるために必要な要素は以下の３つである |
１．双安定 | １．双安定 | ||
２．Cell－Cellコミュニケーション | ２．Cell－Cellコミュニケーション | ||
| - | ３．２つの機能が１つの細胞から実現されること | + | ３．２つの機能が１つの細胞から実現されること--> |
<br>To achieve this “coexistence stability,” our model requires | <br>To achieve this “coexistence stability,” our model requires | ||
| - | <br>1. bistability | + | <br>1. bistability at two distinct states in a single cell |
| - | <br>2. cell-cell communication | + | <br>2. cell-cell communication by quarum sensing |
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| - | それぞれについて以下で説明する。 | + | <!--それぞれについて以下で説明する。--> |
====1. Bistability==== | ====1. Bistability==== | ||
| - | これがないと、動的平衡になってしまう。 | + | <!--これがないと、動的平衡になってしまう。<br>Without this, our model is subject to dynamic equilibrium.--> |
| - | Without this, our model is subject to dynamic equilibrium. | + | '''1-1. At an individual level''' |
| + | |||
| + | For "coexistence stability" in our model, two types of cells should coexist stably. Therefore, cells with '''THE SAME GENE SET''' need to take '''TWO STATE STATES''', A (Worker) and B (Idler) in our project as shown in Fig. 1. To be clearly distinduished from dynamic stability, "coexistence stability" must be achieved by two distinct states unconvertible each other. | ||
[[Image:collective.jpg|thumb|350px| '''Fig.2: Coexistence]] | [[Image:collective.jpg|thumb|350px| '''Fig.2: Coexistence]] | ||
| - | ==== | + | '''1-2. At a collective level''' |
| + | <br> | ||
| + | To achieve "coexistence stability," each cell needs to feel cell types - the ratio of A and B - around. | ||
| + | As is often the case bacteria in the nature, our modified cells here sense the types of coexisting cells by cell-cell communication called quorum sensing. Under the coexistence of A and B at a specific ratio, the whole system (or group) is collectively stable. Fig. 2 simply shows this scheme. | ||
| + | <br> | ||
| + | <br><br> | ||
| + | <br><br> | ||
| + | ====2. Cell-cell communication ==== | ||
| + | When this stable coexistence is disrupted, by removing all the A type for example, these individual cells sense the change. Their states A or B become unstable as if cells became upset - INDIVIDUAL STATES ARE UNSTABLE ⇒ They are COLLECTIVELY UNSTABLE. Then some of them changed their states while the others do not. As a result, at a certain ratio of A and B again, the whole system comes back to a stable states. Here the cells are COLLECTIVELY STABLE ⇒ INDIVIDUALS ARE STABLE under this stable coexistence. | ||
Revision as of 03:46, 24 October 2007
Abstract Concept & Model Requirements Genetic_circuit Works About_our_team
The most important and underlying point of our model is that the system is stable against environmental changes when two or more types of individuals coexist, called "coexistence stability." Our project have aimed at this "coexistence stability," not dynamic equilibrium such as chemical ones
To achieve this “coexistence stability,” our model requires
1. bistability at two distinct states in a single cell
2. cell-cell communication by quarum sensing
1. Bistability
1-1. At an individual level
For "coexistence stability" in our model, two types of cells should coexist stably. Therefore, cells with THE SAME GENE SET need to take TWO STATE STATES, A (Worker) and B (Idler) in our project as shown in Fig. 1. To be clearly distinduished from dynamic stability, "coexistence stability" must be achieved by two distinct states unconvertible each other.
1-2. At a collective level
To achieve "coexistence stability," each cell needs to feel cell types - the ratio of A and B - around.
As is often the case bacteria in the nature, our modified cells here sense the types of coexisting cells by cell-cell communication called quorum sensing. Under the coexistence of A and B at a specific ratio, the whole system (or group) is collectively stable. Fig. 2 simply shows this scheme.
2. Cell-cell communication
When this stable coexistence is disrupted, by removing all the A type for example, these individual cells sense the change. Their states A or B become unstable as if cells became upset - INDIVIDUAL STATES ARE UNSTABLE ⇒ They are COLLECTIVELY UNSTABLE. Then some of them changed their states while the others do not. As a result, at a certain ratio of A and B again, the whole system comes back to a stable states. Here the cells are COLLECTIVELY STABLE ⇒ INDIVIDUALS ARE STABLE under this stable coexistence.
