Tokyo/Works/Assay
From 2007.igem.org
(Difference between revisions)
(→Purpose) |
(→Purpose) |
||
| Line 31: | Line 31: | ||
====Purpose==== | ====Purpose==== | ||
<br>check if LacI represses this hybrid promoter indirectly-measured by IPTG. | <br>check if LacI represses this hybrid promoter indirectly-measured by IPTG. | ||
| + | <br>These parameters(n3,K3) is decided. | ||
<!--<br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | <!--<br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | ||
<br> The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.--> | <br> The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.--> | ||
Revision as of 04:54, 24 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay (Hill function fitting)
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める．
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
1.AHL assay
Purpose
check if AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated in the presence of AHL.
2.IPTG assay
Purpose
check if LacI represses this hybrid promoter indirectly-measured by IPTG.
These parameters(n3,K3) is decided.
Result & Conclusion
2.Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
