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Revision as of 04:57, 24 October 2007 (view source) Nakatani (Talk | contribs) (→Purpose) ← Older edit		Revision as of 05:04, 24 October 2007 (view source) Nakatani (Talk | contribs) (→1.AHL assay) Newer edit →
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-	== [[Tokyo/Repression and activation check with LacI and AHL|1.AHL assay]] ==	+	== [[Tokyo/AHL assay|1.AHL assay]] ==
	====Purpose====		====Purpose====
	check if AHL activates lux-lac hybrid promoter.		check if AHL activates lux-lac hybrid promoter.

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Revision as of 05:04, 24 October 2007

Works top　　0.Hybrid promoter　　1.Formulation　　2.Assay1　　3.Simulation　　4.Assay2　　5.Future works

Purpose of this assay (Hill function fitting)

新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.

1.AHL assay

Purpose

check if AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.

Result & Conclusion

Lux-lac hybrid promoter is activated by AHL.

2.IPTG assay

Purpose

check if LacI represses this hybrid promoter indirectly-measured　by IPTG.
These parameters(n3,K3) is decided.

Result & Conclusion

Lux-lac hybrid promoter is activated by AHL.

2.Preliminary assays

We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.

See more details