Calgary/design
From 2007.igem.org
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| - | <td></td> | + | <td align="right"><a href=""><img img style="border:none" src="https://static.igem.org/mediawiki/2007/2/29/CheckOutevoGEM.gif" alt="Check out evoGEM"></a></td> |
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| - | <td align="center" ><a class="mainLinks" href="https://2007.igem.org/Calgary/choosing_our_project" title="choosing our project" > | + | <td align="center" ><a class="mainLinks" href="https://2007.igem.org/Calgary/choosing_our_project" title="choosing our project" >Projects</a> </td> |
| - | <td align="center" bgcolor="#006633"><a class="mainLinks" href="https://2007.igem.org/Calgary/design" title="designing our project"> | + | <td align="center" bgcolor="#006633"><a class="mainLinks" href="https://2007.igem.org/Calgary/design" title="designing our project">Project Design: Wet Lab</a> </td> |
| - | <td align="center"><a class="mainLinks" href="https://2007.igem.org/Calgary/testing" title="testing our parts and primers" >Testing | + | <td align="center" ><a class="mainLinks" href="https://2007.igem.org/Calgary/constructing_printer" title="constructing our project - printer and software" >Project Design: Printer and Software</a> </td> |
| + | <td align="center"><a class="mainLinks" href="https://2007.igem.org/Calgary/testing" title="testing our parts and primers" >Testing</a> </td> | ||
<td align="center"><a class="mainLinks" href="https://2007.igem.org/Calgary/constructing_wetlab" title="constructing our project" >Constructing our Project: The Wetlab</a> </td> | <td align="center"><a class="mainLinks" href="https://2007.igem.org/Calgary/constructing_wetlab" title="constructing our project" >Constructing our Project: The Wetlab</a> </td> | ||
| - | <td align="center" | + | <td align="center" valign="top"><a class="mainLinks" href="https://2007.igem.org/Calgary/protocols" title="protocols" >Protocols</a> </td> |
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<td align="center"><a class="mainLinks" href="https://2007.igem.org/Calgary/final_result" title="final results" >Final Result of E.co Lisa</a> </td> | <td align="center"><a class="mainLinks" href="https://2007.igem.org/Calgary/final_result" title="final results" >Final Result of E.co Lisa</a> </td> | ||
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Revision as of 21:48, 24 October 2007
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| Projects | Project Design: Wet Lab | Project Design: Printer and Software | Testing | Constructing our Project: The Wetlab | Protocols | Final Result of E.co Lisa |
Once we had chosed our project the next step was to design a system that would do what we wanted. The steps outlined in this section are the MOST CURRENT used in our project. This section does not describe the primers, plates, and parts that were considered but not used. Or the techniques that were attempted but did not work
E. coLisa Project Design
The following circut was designed to implement our system (image and design schematic pending)
E. coLisa Project Preparation Work
Once a design for the project had been determined our team begin preparing all of the materials and reagents needed to complete our project. The idea was to have everything ready to go for when the biobrick parts arrive.
Preparing Plates. AMP resistant
Have everything ready to go for when parts arrive
Quick list of parts...
- R0084 - Light Sensor Promoter
- R0062 - AHL Promoter
- R0011 - Temperature Sensitive Promotor
- S03600 - AHL Intermediate
- I13544 - GFP RBS Terminator
- J23008 - RNA Key
- B0034 - RBS
- B0015 - Terminator
- J06501 - Temperature Sensitive Component
- I51001 - ccdb and AMP resistant (death gene used to do plasmid switches
Wells from registery
I) Rehydrate the wells
II) 2 micro litres of rehydrated part --> transformed into TOP10
