Tokyo/Works/Assay
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== [[Tokyo/AHL assay|1.AHL assay]] == | == [[Tokyo/AHL assay|1.AHL assay]] == | ||
| + | <br>[[Image:hill AHL.JPG|thumb|190px|'''Fig.1''']] | ||
'''Purpose''' | '''Purpose''' | ||
<br>check how AHL activates lux-lac hybrid promoter. | <br>check how AHL activates lux-lac hybrid promoter. | ||
Revision as of 04:12, 25 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay (Hill function fitting)
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
⇒see more details
1.AHL assay
Purpose
check how AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL as shown in Fig. 1.
⇒see more details
2.IPTG assay
Purpose
check how LacI represses this hybrid promoter. In order to adjust LacI concentration, IPTG was added.
These parameters(n3,K3) is decided.
Result & Conclusion
Lux-lac hybrid promoter is activated by AHL.
⇒see more details
Appendix:Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
