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	::::: - 8 μl of insert DNA		::::: - 8 μl of insert DNA
	::::: - 4 μl of vector plasmid		::::: - 4 μl of vector plasmid
-	::::: - 4 μl of Buffer 5X	+	::::: - 4 μl of Buffer 5X [ ]
	::::: - 1 μl of T4 Ligase		::::: - 1 μl of T4 Ligase
-	::::: - 3 μl of mQ water	+	::::: - 3 μl of ultrapure water
	::2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.		::2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.

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Revision as of 09:31, 25 October 2007

(final volume 20 μl)

1. Mix :

- 8 μl of insert DNA

- 4 μl of vector plasmid

- 4 μl of Buffer 5X [ ]

- 1 μl of T4 Ligase

- 3 μl of ultrapure water

2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.

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