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Revision as of 07:08, 25 October 2007 (view source) Nakatani (Talk | contribs) ← Older edit		Revision as of 11:14, 25 October 2007 (view source) Hiro (Talk | contribs) (→Conclusion:) Newer edit →
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	<br>[[Tokyo/Wash |Wash]]		<br>[[Tokyo/Wash |Wash]]
	===Result: ===		===Result: ===
-	===Conclusion: ===	+
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	== ==		== ==
	[[Tokyo/Preliminary assays |"Back to Preliminary assays"]]		[[Tokyo/Preliminary assays |"Back to Preliminary assays"]]

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Revision as of 11:14, 25 October 2007

Contents 1 AHL assay 1.1 Purpose1: 1.2 Samples: 1.3 Procedure: 1.4 Result: 2

AHL assay

Purpose1:

To see how precisely we determine the fluorescence from GFP by removing LB media.

Samples:

placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)

Procedure:

AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash

Result:

"Back to Preliminary assays"