Tokyo/Experimental precision and accurecy
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<br>[[Tokyo/Wash |Wash]] | <br>[[Tokyo/Wash |Wash]] | ||
===Result & conclusion: === | ===Result & conclusion: === | ||
| + | In proportion to the percentages of GFP expressing cells, fluorescence was increased as shown in Fig. 1. | ||
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[[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | ||
Revision as of 11:18, 25 October 2007
Contents |
AHL assay
Purpose1:
To see how precisely we determine the fluorescence from GFP by removing LB media.
Samples:
placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)
Procedure:
AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash
Result & conclusion:
In proportion to the percentages of GFP expressing cells, fluorescence was increased as shown in Fig. 1.
