USTC

From 2007.igem.org

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=== Our Project:<BR>Extensible Logic Circuit in Bacteria ===
=== Our Project:<BR>Extensible Logic Circuit in Bacteria ===
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Logic Artificial Biological Circuit (ABC) and logic Integrated Circuit (IC) are both composed by "components" and "wires". Though we have been enjoying the advantages of Ultra Large Scale IC (ULSI) in modern life, we still cannot implement in biological experiments a somewhat large-scale logic ABC with several levels of logic gates in it.
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Artificial Bio-Logic Circuit is composed by "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale  electronic circuits in modern life, we still cannot implement a somewhat small-scale bio-logic circuit <i>in vivo</i> with several levels of logic gates.
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Our project is to provide a new method for building up a fully extensible logic ABC. For some biological convenience, we are going to produce such biological logic gates as NAND, NOR, NOT which are exactly the basis for modern logic IC. More importantly, several factors  influence the scale of ABC and IC, such as, the size and power of the components, the interference between wires, and standardization of intercommunication. Cis-acting components in ABC may present a smaller scale and power request than trans-acting ones, while the other two factors can be improved by novelly-designed and calibrated wires. We are also going to demonstrate an ABC made by these biological logic gates and wires.
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Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing <i>cis-</i>acting elements can be systematically built up and act as a logic gate, favored for its small scale and potential to implement complicated logic computation <i>in vivo</i>. Meanwhile, artificial repressors with artificial highly-specified DNA-recognition regions are able to transmit signals without mutual interference. In this way, a circuit can be constructed senseless to the number of logic gates and the manner of connections between the wires.
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Such an ABC may not be large, but the important factors we implement in it will naturally make it fully extensible. IC has existed for 50 years, and now how huge its impact is on our modern lives! Then what about the biological logic circuits in the next 50 years? A new world is waiting...
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A demonstration system has also been assembled to show the practicality of this method. Like Digital Electronic Circuits in early days, it is simple and ugly. But how will it appear in futune?
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''Front row:'' [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su XF]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
''Front row:'' [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su XF]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
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Revision as of 12:24, 25 October 2007


USTC Logo.png

Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed by "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale bio-logic circuit in vivo with several levels of logic gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing cis-acting elements can be systematically built up and act as a logic gate, favored for its small scale and potential to implement complicated logic computation in vivo. Meanwhile, artificial repressors with artificial highly-specified DNA-recognition regions are able to transmit signals without mutual interference. In this way, a circuit can be constructed senseless to the number of logic gates and the manner of connections between the wires.

A demonstration system has also been assembled to show the practicality of this method. Like Digital Electronic Circuits in early days, it is simple and ugly. But how will it appear in futune?


DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Project

Introduction


Core Logic Components:

Logic-Gate Promoters


Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico


Peripheral Devices:

Inputs and Outputs


Extensible System:

A Demonstration

Further More


Events

19/09/2007
We are pummeled by [http://news.yahoo.com/s/ap/20070919/ap_on_re_as/asia_typhoon Typhoon Wipha]. Most of our service providers suspend their work. What a sad day!

18/09/2007
Draft of our schedule for the Jamboree.

04/09/2007
The second scheme of NAND gate has been built up and the rough data show that it works. More precise quantitative data is to be measured.

02/09/2007
Double-reporters system version 2 has passed sequencing, and been confirmed by X-gal assay and fluorescence observation.

Previous

Members

Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu


Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun



Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]

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