Chiba/Communication
From 2007.igem.org
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===Sender=== | ===Sender=== | ||
*ptet-luxI( (use BBa_...) | *ptet-luxI( (use BBa_...) | ||
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==Experiments== | ==Experiments== | ||
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*調べるwhether does it change or not when the AHL precursur, methionine, is added. Right we show the AHL synthesis pathway. | *調べるwhether does it change or not when the AHL precursur, methionine, is added. Right we show the AHL synthesis pathway. | ||
====Procedure==== | ====Procedure==== | ||
| + | [[Image:Chiba_senderassay.jpg]] | ||
====Result==== | ====Result==== | ||
| + | [[Image:AHL_test_photo.jpg]] | ||
Revision as of 06:55, 26 October 2007
|
Introduction | Project Design | Bacteria Linker | Communication | Size Controller | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Communication: Our Aim
Fig1.
違う遺伝子回路を持つ２つの細胞（sender&receiver）に分ける．あらかじめお互い結合しているSenderが，周りのreceiverにシグナルを与えてstickey handsを生やす．そして合体．このシグナルにはQuorum sensingを利用する．
About Quorum Sensing
Fig2.
クオラムセンシングとは，バクテリアが細胞間で行う（細胞密度に依存する）シグナル伝達のシステムである（＜この辺wikipediaから引用する）．自然界ではバクテリア（限定したほうが良い？）は常にAHLを合成している．AHLの濃度がある値以上になる，すなわち細胞密度がある値を超えるとAHL依存性プロモータ（LuxP）のスイッチがオンになり，遺伝子が発現される．
詳しい遺伝子回路は図に示す通り．
Our Construct
Recievers
- ver1.
- ptet-luxr-plux-gfp-aiia
- ver2.
- ptet-luxr-plux-gfp-inv-aiia
Sender
- ptet-luxI( (use BBa_...)
Experiments
Sender Assay
Purpose
- 調べるthe absolute number of senders required to activate pLux.
- 調べる the difference in synthesis ability among Strains.
- 調べるwhether does it change or not when the AHL precursur, methionine, is added. Right we show the AHL synthesis pathway.
Procedure
Result
