Freiburg07/In vivo test I

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<td width="131">IPTG [µg/ml]</td>
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<td width="131">IPTG [M]</td>
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<td>0.5</td>
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<td>1</td>
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<td>0.5</td>
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<td>1</td>
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<td>0.5</td>
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<td>1</td>
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<td>0.5</td>
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<td>1</td>
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Latest revision as of 08:47, 26 October 2007

In vivo test I

Ca2+ stock:        100mM

Ca2+  [µg/ml] 0 0.1 1.0 10
IPTG [M] 1 1 1 1
Amp [µg/ml] 50 50 50 50

With this concentration of Ca2+, Amp and IPTG were following plates pour.

3 plates IPTG

3 plates IPTG + AMP

3 plates IPTG + Amp + Ca2+ (0.1)

3 plates IPTG + Amp + Ca2+ (1.0)

3 plates IPTG + Amp + Ca2+ (10)

3 plates IPTG + Ca2+ (0.1)

3 plates IPTG + Ca2+ (1.0)

3 plates IPTG + Ca2+ (10)

500 cells/100µl were plated in each plate.


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