Cambridge
From 2007.igem.org
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[[Image:Camb amplif icon.png|50px|left]] | [[Image:Camb amplif icon.png|50px|left]] | ||
| - | <span style="font-weight: bold; font-size: 120%; line-height: 300%;">PoPS amplifier</span> | + | <span style="font-weight: bold; font-size: 120%; padding: 0 0 0 0.5em; line-height: 300%;">PoPS amplifier</span> |
We built a standard amplifier which can be used in any synthetic transcriptional system, taking in a standard PoPS input and giving a PoPS output of a known amplification factor. In addition, the activator-promoter pairs tested have characterised crosstalk giving the potential for a controlled multifaceted response. | We built a standard amplifier which can be used in any synthetic transcriptional system, taking in a standard PoPS input and giving a PoPS output of a known amplification factor. In addition, the activator-promoter pairs tested have characterised crosstalk giving the potential for a controlled multifaceted response. | ||
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[[Image:Camb agr icon.png|50px|left]] | [[Image:Camb agr icon.png|50px|left]] | ||
| - | <span style="font-weight: bold; font-size: 120%; line-height: 300%;">Peptide signalling</span> | + | <span style="font-weight: bold; font-size: 120%; padding: 0 0 0 0.5em; line-height: 300%;">Peptide signalling</span> |
The Accessory Gene Regulator (Agr) system in ''S. aureus'' is an oligopeptide-based quorum sensing mechanism. We worked on converting it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (''B. subtilis'') and Gram-negative bacteria (''E. coli''), based on controllable secretion and detection of the signalling peptide AIP. | The Accessory Gene Regulator (Agr) system in ''S. aureus'' is an oligopeptide-based quorum sensing mechanism. We worked on converting it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (''B. subtilis'') and Gram-negative bacteria (''E. coli''), based on controllable secretion and detection of the signalling peptide AIP. | ||
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[[Image:Camb bacillus icon.png|50px|left]] | [[Image:Camb bacillus icon.png|50px|left]] | ||
| - | <span style="font-weight: bold; font-size: 120%; line-height: 300%;">Gram-positive chassis</span> | + | <span style="font-weight: bold; font-size: 120%; padding: 0 0 0 0.5em; line-height: 300%;">Gram-positive chassis</span> |
Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We worked on constructing a "chassis" out of ''Bacillus subtilis'', a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits. | Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We worked on constructing a "chassis" out of ''Bacillus subtilis'', a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits. | ||
Revision as of 15:45, 26 October 2007
 BOL: Bacteria OnLine | ||
IntroductionIn order to engineer interesting and useful functions in biology, a robust and extensive range of inter- and intra-cellular signalling pathways must be available. By analogy with the Internet, such a system must allow communication between cells of different heritage and structure (different “operating systems”). In the course of this project we attempted to implement candidates for both types of signalling mechanism, and additionally made progress towards adding a new Gram-positive platform for synthetic biology to the Registry. ???? MORE BLURB / PICS
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 PoPS amplifier We built a standard amplifier which can be used in any synthetic transcriptional system, taking in a standard PoPS input and giving a PoPS output of a known amplification factor. In addition, the activator-promoter pairs tested have characterised crosstalk giving the potential for a controlled multifaceted response. |
 Peptide signalling The Accessory Gene Regulator (Agr) system in S. aureus is an oligopeptide-based quorum sensing mechanism. We worked on converting it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (B. subtilis) and Gram-negative bacteria (E. coli), based on controllable secretion and detection of the signalling peptide AIP. |
 Gram-positive chassis Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We worked on constructing a "chassis" out of Bacillus subtilis, a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits. |
Meet the team!
 Top row, from left: James Brown (PhD student mentor), Jim Haseloff (faculty supervisor), Gos Micklem (faculty supervisor) Second row, from left: Yi Jin Liew, John Crowe (faculty), Lovelace Soirez, Stephanie May, Yue Miao Third row, from left: Zhizhen Zhao (Jane), Stefan Milde, Dmitry Malyshev, Xinxuan Soh (Sheila) Front row, from left: Narin Hengrung, Yi Han, David Wyatt Not in photo: Jim Ajioka (faculty supervisor), Lorenz Wernisch (faculty), Tony Southall (PhD student) The mailing list for the whole Cambridge iGEM2007 team is igem2007 [at] ccbi.cam.ac.uk | ||
SponsorsWe are extremely grateful to the following organisations for their support of our project: Major sponsors
Other sponsors
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