Chiba/Project Design
From 2007.igem.org
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#Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.<br> | #Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.<br> | ||
#This way, cluster and grow like a snowball. At the same time, receivers degrade AHL and thus limit the space where AHL reaches. By controlling the rate of AHL degradation, one can define the size of bacterial culster, MARIMO. | #This way, cluster and grow like a snowball. At the same time, receivers degrade AHL and thus limit the space where AHL reaches. By controlling the rate of AHL degradation, one can define the size of bacterial culster, MARIMO. | ||
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Revision as of 16:04, 26 October 2007
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Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Project Design
Concept
(photo taken from 社団法人 農林水産技術情報協会http://www.afftis.or.jp/index.html)
We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other.
What our system requires
1.Sticky TagMake a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds with metal ions) on the flagellar fillament.  Fig1. His-tagged flagella as a bactria linker. 鞭毛は便宜上１本しか描いてないが実際は1~10本/細胞． |
2.CommunicationMake a Bacterial Communication. 違う遺伝子回路を持つ２つの細胞（sender&receiver）に分ける．あらかじめお互い結合しているSenderが，周りのreceiverにシグナルを与えてsticky handsを生やす．そして合体．  Fig2. Bacterial Communication. |
3.Size ControlMake an AHL concentration gradient for quorum sensing.  Fig3. AHL concentration gradient. |
How Our System Works
- Senders stick to each other by way of metal ions using flagella displaying histidine tags. This becomes the core of Marimo. They also produce AHL to call receiver cells.
- Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders (core).
- Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.
- This way, cluster and grow like a snowball. At the same time, receivers degrade AHL and thus limit the space where AHL reaches. By controlling the rate of AHL degradation, one can define the size of bacterial culster, MARIMO.
