Tokyo/Works/Assay
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<br>[[Tokyo/Works|Works top]] 0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]] 1.[[Tokyo/Works/Formulation |Formulation]] 2.[[Tokyo/Works/Assay |Assay1]] 3.[[Tokyo/Works/Simulation |Simulation]] 4.[[Tokyo/Works/Assay2 |Assay2]] 5.[[Tokyo/Works/Future works |Future works]] | <br>[[Tokyo/Works|Works top]] 0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]] 1.[[Tokyo/Works/Formulation |Formulation]] 2.[[Tokyo/Works/Assay |Assay1]] 3.[[Tokyo/Works/Simulation |Simulation]] 4.[[Tokyo/Works/Assay2 |Assay2]] 5.[[Tokyo/Works/Future works |Future works]] | ||
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| - | [[Tokyo/Hill function fitting |Purpose of this assay]] [[Tokyo/AHL assay| | + | [[Tokyo/Hill function fitting |Purpose of this assay]] [[Tokyo/AHL assay|Effect of AHL]] [[Tokyo/IPTG assay|2.IPTG assay]] [[Tokyo/Preliminary assays |Preliminary assays]] |
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| - | == [[Tokyo/AHL assay| | + | == [[Tokyo/AHL assay|Effect of AHL]] == |
<br>[[Image:hill AHL.JPG|thumb|210px|'''Fig.1 The result of AHL assay''' Fluorescence intensity (arbitrary unit, a.u.) as a function of the concentration of AHL was determined. ]] | <br>[[Image:hill AHL.JPG|thumb|210px|'''Fig.1 The result of AHL assay''' Fluorescence intensity (arbitrary unit, a.u.) as a function of the concentration of AHL was determined. ]] | ||
'''Purpose''' | '''Purpose''' | ||
Revision as of 18:20, 26 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay Effect of AHL 2.IPTG assay Preliminary assays
Purpose of this assay (Hill function fitting)
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
⇒see more details
Effect of AHL
Purpose
check how AHL activates lux-lac hybrid promoter.
These parameters(n2,K2) is decided.
Result & Conclusion
As the concentration of AHL increases, GFP fluorescence increased, indicating that the hybrid promoter’s activation is strengthend with increasing concentration of AHL. From the activation graph in Fig. 1, the characteristics of the hybrid promoter expressed in Hill function, such as (n2,K2), is determined.
⇒see more details
2.IPTG assay
Purpose
check how LacI represses this hybrid promoter. In order to adjust LacI repression, IPTG was added.
These parameters(n3,K3) is decided.
Result & Conclusion
The concentration of IPTG dose-denpendently increased GFP fluorescence. It indicates that the hybrid promoter in the cells expressing LacI became increasingly strengthened by decline of repression by LacI. The activation graph in Fig. 2, the characteristics of the hybrid promoter expressed in Hill function is determined.
⇒see more details
Appendix: Preliminary assays
We conducted preliminary assays as practice experiments and check of materials and methods.
⇒See more details
Before(Formulation) << Assay1 >> Assay1 contents >>>> Simulation
