Chiba/Communication
From 2007.igem.org
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| - | ==Communication | + | ==Communication== |
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[[Image:Chiba_prj_com.png|frame|'''Fig1.''' Micro.]] | [[Image:Chiba_prj_com.png|frame|'''Fig1.''' Micro.]] | ||
Our aim is that bacterias stick around the bacterias core. We make bacteria sending a signal(sender) and bacteria receiving a signal(receiver). Senders sticking each other in advance send a signal to receivers, which grow stickey hands. In this way, bacterias stick. We use Quorum sensing as a signal.<br> | Our aim is that bacterias stick around the bacterias core. We make bacteria sending a signal(sender) and bacteria receiving a signal(receiver). Senders sticking each other in advance send a signal to receivers, which grow stickey hands. In this way, bacterias stick. We use Quorum sensing as a signal.<br> | ||
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<br clear="all"> | <br clear="all"> | ||
| - | ==About Quorum Sensing== | + | ===About Quorum Sensing=== |
[[Image:Chiba_aboutqs.png|frame|'''Fig2.''']] | [[Image:Chiba_aboutqs.png|frame|'''Fig2.''']] | ||
Quorum Sinsing is cell-to-cell signaling action of bacteria. | Quorum Sinsing is cell-to-cell signaling action of bacteria. | ||
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[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing] | [http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing] | ||
| - | == | + | ==Experiments== |
| - | ===Recievers=== | + | ===Making Recievers=== |
====We Made==== | ====We Made==== | ||
*ver.1(BBa_T729006) | *ver.1(BBa_T729006) | ||
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**[[Image:Rec inv aiia.jpg|357x40px]] | **[[Image:Rec inv aiia.jpg|357x40px]] | ||
| - | === | + | ===Making Senders=== |
====We Made==== | ====We Made==== | ||
*MetK Sender | *MetK Sender | ||
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futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro | futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro | ||
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==Experiments== | ==Experiments== | ||
===Sender Assay=== | ===Sender Assay=== | ||
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1.Senderは少なくとも5x10^4は必要である。 | 1.Senderは少なくとも5x10^4は必要である。 | ||
2.Senderの株によってLuxIの活性が違う。 | 2.Senderの株によってLuxIの活性が違う。 | ||
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Revision as of 19:54, 26 October 2007
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Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Communication
Our Aim
Our aim is that bacterias stick around the bacterias core. We make bacteria sending a signal(sender) and bacteria receiving a signal(receiver). Senders sticking each other in advance send a signal to receivers, which grow stickey hands. In this way, bacterias stick. We use Quorum sensing as a signal.
違う遺伝子回路を持つ２つの細胞（sender&receiver）に分ける．あらかじめお互い結合しているSenderが，周りのreceiverにシグナルを与えてstickey handsを生やす．そして合体．このシグナルにはQuorum sensingを利用する．
About Quorum Sensing
Quorum Sinsing is cell-to-cell signaling action of bacteria. Detect the cell density of same species and coordinating the behavior of cells within a population. Species of Gram-Negativfe signaling information transfer molecule (autoinducer) is acyl homoserine lactone (AHL).When AHL concentration exceed threshold thus the cell population over a certain number, AHL regulated promoter is activated transcription.
Detail geneticaly circuit discribed in Fig2.
クオラムセンシングとは，バクテリアが細胞間で行うシグナル伝達のシステムである．自分と同種の菌の生息密度を感知して、それに応じて物質の産生をコントロールする．情報伝達に使用している化合物（オートインデューサー）はグラム陰性菌の場合AHLである．AHLの濃度がある値以上になる，すなわち細胞密度がある値を超えるとAHL依存性プロモータ（LuxP）のスイッチがオンになり，遺伝子が発現される．
詳しい遺伝子回路は図に示す通り．
- more about quorum sensing
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]
Experiments
Making Recievers
We Made
- ver.1(BBa_T729006)
- ver2.(BBa_I729005)
From Biobrick
- ver.0(BBa_T9002)
Future Work
- ver3
Making Senders
We Made
- MetK Sender
From Biobrick
- Sender(BBa_S03623)
Future Work
- Light Sensing Sender (Light -> AHL)
futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro
