Tokyo/Preliminary assays

From 2007.igem.org

(Difference between revisions)
Line 2: Line 2:
<br>[[Tokyo/Works|Works top]]  0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]  1.[[Tokyo/Works/Formulation |Formulation]]  '''2.[[Tokyo/Works/Assay |Assay1]]'''  3.[[Tokyo/Works/Simulation |Simulation]]  4.[[Tokyo/Works/Assay2 |Assay2]]  5.[[Tokyo/Works/Future works |Future works]]
<br>[[Tokyo/Works|Works top]]  0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]  1.[[Tokyo/Works/Formulation |Formulation]]  '''2.[[Tokyo/Works/Assay |Assay1]]'''  3.[[Tokyo/Works/Simulation |Simulation]]  4.[[Tokyo/Works/Assay2 |Assay2]]  5.[[Tokyo/Works/Future works |Future works]]
<br><br>
<br><br>
-
[[Tokyo/Hill function fitting |Objective of this assay]]  [[Tokyo/AHL assay|Effect of AHL]]  [[Tokyo/IPTG assay|Effect of IPTG]]  [[Tokyo/Preliminary assays |Preliminary assays]]
+
[[Tokyo/Hill function fitting |Objective of this assay]]  [[Tokyo/AHL assay|Effect of AHL]]  [[Tokyo/IPTG assay|Effect of LacI-IPTG]]  [[Tokyo/Preliminary assays |Preliminary assays]]
== ==
== ==

Revision as of 20:58, 26 October 2007


Works top  0.Hybrid promoter  1.Formulation  2.Assay1  3.Simulation  4.Assay2  5.Future works

Objective of this assay  Effect of AHL  Effect of LacI-IPTG  Preliminary assays

Preliminaly assays were conducted
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

AHL preparation

Experimental precision and accurecy

AHL assay practice

Lux-lac hybrid promoter activation check

OD correction

Wash

New AHL check