Chiba/Project Design
From 2007.igem.org
(Difference between revisions)
(→Project Design) |
|||
| Line 11: | Line 11: | ||
==Project Design== | ==Project Design== | ||
===Concept=== | ===Concept=== | ||
| - | [[Image:Story.PNG|300x300px | + | [[Image:Story.PNG|300x300px]]<br> |
We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other. | We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other. | ||
| Line 20: | Line 20: | ||
Make a '''His-tagged Flagella'''. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.<br> | Make a '''His-tagged Flagella'''. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.<br> | ||
| - | [[Image:Chiba_stickbacteria.png|frame|left|''' | + | [[Image:Chiba_stickbacteria.png|frame|left|'''Fig2. ''' His-tagged flagella as a bactria linker. This image depicts only one tail as flagella (the real bacteria have about ten flagella per cell).]]<br> |
| width="30%" valign="top" | | | width="30%" valign="top" | | ||
| Line 26: | Line 26: | ||
Make a '''Bacterial Communication'''. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.<br> | Make a '''Bacterial Communication'''. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.<br> | ||
| - | [[Image:Chiba_prj_com.png|frame|left|''' | + | [[Image:Chiba_prj_com.png|frame|left|'''Fig3.''' Bacterial Communication.]] |
| width="30%" valign="top" | | | width="30%" valign="top" | | ||
====[[Chiba/Quorum_Sensing|3.Size Control]]==== | ====[[Chiba/Quorum_Sensing|3.Size Control]]==== | ||
Make an '''AHL localized region''' for quorum sensing. | Make an '''AHL localized region''' for quorum sensing. | ||
| - | [[Image:Chiba_proj_ahlconc.png|frame|left|''' | + | [[Image:Chiba_proj_ahlconc.png|frame|left|'''Fig4.''' Controlling AHL diffusing area and the size of Bacteria Marimo.]] |
|} | |} | ||
Revision as of 04:44, 27 October 2007
|
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal |
Project Design
Concept
We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other.
What our system requires
1.Affinity TagMake a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.  Fig2. His-tagged flagella as a bactria linker. This image depicts only one tail as flagella (the real bacteria have about ten flagella per cell). |
2.Communication ModuleMake a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.  Fig3. Bacterial Communication. |
3.Size ControlMake an AHL localized region for quorum sensing.  Fig4. Controlling AHL diffusing area and the size of Bacteria Marimo. |
How Our System Works
- Senders whose flagella display the histidine tags stick to each other through metal ions. This becomes the core of Bacteria Marimo. The senders also produce AHL to sign the receiver cells.
- Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders core.
- Receivers stick to the senders core and themselves through metal ions.... one after another.
- As seen, the cluster grows like a snowball. At the same time, the receivers degrade AHL and thus AHL diffusion space around the mixed cluster limited. By controlling the rate of AHL degradation and so on, one can define the size of such Bacteria Marimo.
