Tokyo/Preliminary assays

From 2007.igem.org

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== ==
== ==
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'''Preliminaly assays were conducted'''
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'''Preliminary assays were conducted'''
<br>- '''to practice and experience the assays with AHL and IPTG'''
<br>- '''to practice and experience the assays with AHL and IPTG'''
<br>- '''to check if and how our materials actually worked.'''
<br>- '''to check if and how our materials actually worked.'''

Revision as of 05:10, 27 October 2007


Works top   0.Hybrid promoter   1.Formulation  2.Assay1   3.Simulation   4.Assay2   5.Future works

Objective of this assay   Effect of AHL   Effect of LacI-IPTG  Preliminary assays

Preliminary assays were conducted
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

AHL preparation

Experimental precision and accurecy

AHL assay practice

Lux-lac hybrid promoter activation check

OD correction

Wash

New AHL check