OtsA-construction file

From 2007.igem.org

(Difference between revisions)
 
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PCR ThpA F/R on ThpA          (553bp, BglII/XhoI)<br>
+
PCR otsAF/otsAIR on otsA    (413 bp, gp=A)<br>
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Sub into PBca1144                  (BglII/XhoI, 2054+910  L)<br>
+
PCR otsAIF/otsAR on otsA    (1077 bp, gp=B)<br>
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Product is pBca9145-BBa I716331      <br>
+
--------------------------------------------
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---------------------------------------------------------------------------------------------<br>
+
PCR otsAF/otsAR on A+B      (1456 bp BglII/XhoI)<br>
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ThpAF  Forward BglII site anneals to ThpA cgtacAGATCTatgaccgccgcaccagcagattttgcccgtgcccgaagcgaGttcctcagtatcg<br>
+
Digest pBca9145-Bca1144      (BglII/XhoI 2054+910, L)<br>
-
<br>
+
Product pBca9145-BBa I716301<br>
-
 
+
--------------------------------------------<br>
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ThpAR  Reverse XhoI site anneals to ThpA anneals to XhoI cgcatctcgagtcaggtggcgaacgggccta
+
otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC<br>
 +
otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG<br>
 +
otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG<br>
 +
otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC<br>

Latest revision as of 17:57, 2 July 2007

PCR otsAF/otsAIR on otsA (413 bp, gp=A)
PCR otsAIF/otsAR on otsA (1077 bp, gp=B)

PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI)
Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L)
Product pBca9145-BBa I716301


otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC
otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG
otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG
otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC

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