From 2007.igem.org

---

Revision as of 23:30, 3 July 2007

8   Forward primer.   
cgacgAGATCTgctaacacgtaaactccac

9   Reverse to get the RBS, and an ATG (what they did)
gcgtgCTCGAGttaGGATCCcattgcttttcctt

10  Reverse to get without an RBS (unsure if this will work)
gcataCTCGAGttaGGATCCgccattgaatacctgtcca

13  Forward get RFP w/o start codon
cgacgAGATCTgcttcctccgaagacgttatc

14  Reverse get RFP
gcataCTCGAGttaGGATCCttattaagcaccggtggag



pcr 7/3

Template for all: I716004


1) ay008/9      349bp
2) ay008/10     333bp
3) ay011/12     709bp