Paris/July 5

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  • Culture of 150 ml of MG1655.

At OD = 0.2 : Centrifugation of 25ml of culture, filtration of the supernatant. ==> S0.2
Same thing at OD= 0.4 , 0.6 and 0.8 , leading to the solutions: S0.2, S0.4, S0.6 and S0.8

  • Transduction of the DapA deletion into MG1655 failed... We will redo the stock

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