Melbourne/primary Restriction enzymes
From 2007.igem.org
(Difference between revisions)
| Line 26: | Line 26: | ||
!EcoRI | !EcoRI | ||
|5G^AATTC3 | |5G^AATTC3 | ||
| - | | | + | |B1,2,3&4 |
|US$53 | |US$53 | ||
|10,000 @ 20,000U/ml | |10,000 @ 20,000U/ml | ||
| Line 35: | Line 35: | ||
!HaeII | !HaeII | ||
|5RGCGC^Y3 | |5RGCGC^Y3 | ||
| - | | | + | |B4 & B2 |
|US$53 | |US$53 | ||
|2,000U @ 20,000U/ml | |2,000U @ 20,000U/ml | ||
| Line 44: | Line 44: | ||
!SalI | !SalI | ||
|5G^TCGAC3 | |5G^TCGAC3 | ||
| - | | | + | |B3 |
|US$56 | |US$56 | ||
|2,000U @ 20,000U/ml | |2,000U @ 20,000U/ml | ||
| Line 53: | Line 53: | ||
!XbaI | !XbaI | ||
|5T^CTAGA3 | |5T^CTAGA3 | ||
| - | | | + | |B2 |
|US$63 | |US$63 | ||
|3,000U @ 20,000U/ml | |3,000U @ 20,000U/ml | ||
Revision as of 06:54, 11 July 2007
<return to list of primary reagents> <team home page>
Contents |
Physical data
- Description: NEB Restriction enzymes are used generally in the Lab.
| Enzyme | Cut site | 100% buffers | Cost | No of U | Part no/data | location | In store |
|---|---|---|---|---|---|---|---|
| PstI | 5CTGCA^G3 | US$58 | 10,000 @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0140S] | YES | ||
| EcoRI | 5G^AATTC3 | B1,2,3&4 | US$53 | 10,000 @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0101.asp NEB.R0101S] | YES | |
| HaeII | 5RGCGC^Y3 | B4 & B2 | US$53 | 2,000U @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0107.asp NEB.R0107S] | YES | |
| SalI | 5G^TCGAC3 | B3 | US$56 | 2,000U @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0138.asp NEB.R0138S] | YES | |
| XbaI | 5T^CTAGA3 | B2 | US$63 | 3,000U @ 20,000U/ml | [http://www.neb.com/nebecomm/products/productR0145.asp NEB.R0145S] | YES |
Double Digest Buffers
Lab Location
- Location see table
- Permision to use required? No
Store
- Available from Bio21 store? Yes
- location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see Daisy).
Manufacturer:
- Manufacturer:NEB
- complete listing of available enzymes
- PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007
