Paris/Oligos

From 2007.igem.org

Revision as of 10:08, 13 July 2007

Constructions

• Lox71-FtsZ:

Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89

FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49

• Lox71-FtsA-FtsZ:

Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89

• Deletion of Ecor1 site in FtsZ:

DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40

DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40

• Lox66-DapAColi:

Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84

DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52

• Lox66-DapASubtilis:

Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84

DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52

• Lox71-KmR-Lox66:

Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85

Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86

• Deletion of Pst1 site in DGAT:

DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40

DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40