Paris/July 14
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David.bikard (Talk | contribs) (→What to do ?) |
Eismoustique (Talk | contribs) (→What to do ?) |
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* Digestion of the purifications products with appropriate enzymes | * Digestion of the purifications products with appropriate enzymes | ||
* Purification | * Purification | ||
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| + | == What has been done == | ||
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| + | * Several colonies of transduced MG1655 are obtained (transduction from dapA- strain w121): | ||
| + | ** Isolation of 10 different colonies on LB Citrate Erythro DAP plates | ||
Revision as of 09:39, 14 July 2007
What to do ?
- Remove the plate of the transduction experiment and the transformation ones from the 37°C incubator (Upper-left shelf): If it has worked and if someones comes on Sunday:
- isolation of the clones from the transduction on petri-dishes (LB+DAP+Erm+Citrate)
- LB + antibio culture of the transformants (to make a glycerol stock and to do MiniPreps)
- Make a gel (0.8%) and migrate the PCR products
- Purify them
- Do the assembly PCR
If enough time (we can also wait to have the plasmids to do everything at the same time):
- Digestion of the purifications products with appropriate enzymes
- Purification
What has been done
- Several colonies of transduced MG1655 are obtained (transduction from dapA- strain w121):
- Isolation of 10 different colonies on LB Citrate Erythro DAP plates
