From 2007.igem.org

Back To Main Page | Back To Wetlab Log

---

Contents 1 Week 8 1.1 Monday 20th August 2007 1.2 Tuesday 21st August 2007 1.3 Wednesday 22nd August 2007 1.4 Thursday 23rd August 2007

Week 8

Monday 20th August 2007

1. Chrsitine did more minipreps for colonies suspected of containing the 7 gene operon. When these minipreps were run on a gel there was no evidence of an insert.

1. These
2. are
   1. numbered
3. sections

This is	a table
Look at the code	to see the template.

Tuesday 21st August 2007

1. Maija digested the construction vectors 3/20G and 4/6B, and the 7 gene operon with SpeI and XbaI (Protocol 7) with the intention of then ligating the 7 gene operon into the construction vectors. The 7 gene operon DNA (taken from sample 2 of the PCR which was gel extracted 13/08/07) did not appear on the gel.

   Label	Description	Enzymes	Expected size	Result
   3/20G	Construction vector	SpeI, XbaI	2071bp	2071bp
   4/6B	Construction vector	SpeI, XbaI	2928bp	2928bp
   (*a→g*)	7 gene operon	SpeI, XbaI	6263bp	No result

2. Christine gel extracted 4/6B and 3/20G (see Protocol 11) using 30ul ddH2O at 60°C to elute.

   Wednesday 22nd August 2007

   Thursday 23rd August 2007

   === Friday 24th August 2007 ===