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From 2007.igem.org
Using Matlab we simulated the behavior of the system when o.a. concentration varies. Then, we plotted the steady state values of PHO80PHO85 (red light) and PHO8 (green light). We started by examining the results using the first couple of input function, i.e. saturating at The following pictures show the results of this analysis when K1 varies.
It's clear that k1=0.1 and K1=0.5 doesn't ensure a correct behavior of the system: the repression of PHO8 is too weak when the oleic acid concentration becomes greater than 7.1 mM! We repeated the same analysis for the varying parameters K2 and K3 and we found that a good behavior is given by k2=1 and k3=0.1. This is confirmed be the following pictures: