From 2007.igem.org

Preparation instructions:

1.Dissolve 56.4g in 1L of distilled water.

2.Autoclave for 15min at 121°C.

This convenient 5x concentrate can be stored and diluited as needed to prepare 5L of 1x M9 minimal salts. For M9 minimal medium:

1.Aseptically dilute 200mL of M9 minimal salts, 5x concentrate with 800mL of sterile water, if necessary, cool to 45-50°C.

2.Aseptically add 20mL of sterile 1M glucose and 2mL of sterile 1M magnesium solfate to prepare 1L of M9 minimal medium.

3.If desired aseptically add 0.1mL of 1M sterile calcium chlorid to the M9 minimal medium. The M9 minimal medium may also be supplemted with the appropriate amino acids.

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