| Sample | Date | Plasmid | Clone | Primer | Result | File Link
|
| AY001 | 060407 | pBca9145-Bca1145 | #1 | ca998 | Probably good. Long, ambiguous long AAAAAAA. | AY001
|
| AY002 | 060407 | pBca9145-Bca1145 | #2 | ca998 | Good. | AY002
|
| AY003 | 061207 | pBca9145-HPI/katG (I716???) | A1 | ca998 | super wrong. Seems like only half of HPI/katG, from the AY004.Sal.katG.HPI-Fx "mutate GAT>GAC (BglII)" til the end, was cloned. | AY003
|
| AY004 | 061207 | pBca9145-HPI/katG (I716???) | A3 | ca998 | generally OK. There is one extra G at 584 in the goal plasmid in the calls file. There is low confidence, but I am not sure what to make of it. Possibly should send for recheck at this point; may be necessary to check the mutation point @1308, and the end portion as well. But based on the miniprep digest run yesterday, this guy has a high chance of being the right one. DECISION: sent as AY007 with G01001 for a reverse check. | AY004
|
| AY005 | 061207 | pBca9145-HPI/katG (I716???) | A4 | ca998 | crazy :( there are big gaps | AY005
|
| AY006 | 061207 | pBca9145-wbbL (I716000) | B1 | ca998 | also crazy | AY006
|
| AY007 | 061307 | pBca9145-HPI/katG (I716???) | A3 | G01001 | Looks good actually, only notable thing is silent point mutation @ 1782 TCG > TCA still S. but the mutation point was just missed by sequencing. I don't think it's necessary to recheck; i have faith the primers worked right. well.. idk. major concern still is the possible addition mutation of a G | AY007
|
| AY008 | 061307 | pBca9145-neuS (I716001) | C1 | ca998 | Forward. Looks great! | AY008
|
| AY009 | 061307 | pBca9145-neuS (I716001) | C1 | G01001 | Reverse. Looks great! | AY009
|
| AY010 | ??? | ??? | ??? | ??? | ??? | AY010
|
| AY011 | ??? | ??? | ??? | ??? | ??? | AY011
|
| AY012 | ??? | ??? | ??? | ??? | ??? | AY012
|
| AY013 | ??? | ??? | ??? | ??? | ??? | AY013
|
| AY014 | ??? | ??? | ??? | ??? | ??? | AY014
|
| AY015 | ??? | ??? | ??? | ??? | ??? | AY015
|
| AY016 | ??? | ??? | ??? | ??? | ??? | AY016
|
| AY017 | ??? | ??? | ??? | ??? | ??? | AY017
|
| AY018 | ??? | ??? | ??? | ??? | ??? | AY018
|
| AY019 | ??? | ??? | ??? | ??? | ??? | AY019
|
| AY020 | ??? | ??? | ??? | ??? | ??? | AY020
|
| AY021 | ??? | ??? | ??? | ??? | ??? | AY021
|
| AY022 | ??? | ??? | ??? | ??? | ??? | AY022
|
| AY023 | ??? | ??? | ??? | ??? | ??? | AY023
|
| AY024 | ??? | ??? | ??? | ??? | ??? | AY024
|
| AY025 | ??? | ??? | ??? | ??? | ??? | AY025
|
