USTC

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Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing cis-acting elements and favored for its small scale and potential to implement complicated logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specified DNA-recognition regions are able to transmit signals without mutual interference, just as copper wires. In this way, a circuit can be constructed regardless of the number of logic gates and the manner of connections between the wires.

A demonstration system has also been assembled to show the practicality of this method.Just like Digital Electronic Circuits in early days, it is simple and ugly. But how will it appear in future?

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian

Project

Introduction

Core Logic Components:

Logic-Gate Promoters

Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico

Peripheral Devices:

Inputs and Outputs

Extensible System:

A Demonstration

Further More

Statistics

4mL/tube LB medium consumption
> 1400 tubes

18/09/2007
Draft of our schedule for the Jamboree.

04/09/2007
The second scheme of NAND gate has been built up and the rough data show that it works. More precise quantitative data is to be measured.

02/09/2007
Double-reporters system version 2 has passed sequencing, and been confirmed by X-gal assay and fluorescence observation.