Tianjin/FLIP-FLOP

Design

"Flip-flop" is the common name given to two-state devices which offer basic memory for sequential logic operations. Flip-flops are widely used for digital data storage and transfer as well as in banks called "registers" for the storage of binary numerical data. Based on the conception of "Flip-flop" and synthetic biology, we designed the Genetically RS FLIP-FLOP whose output signal is regulated by additional input signal. Besides this, we modulate the performance of Genetically RS FLIP-FLOP to optimize our original design.

1.Introduction to the logic rules of our flip-flop


The logic principle of our design is shown above. Unless the input signal transfer from one stable condition to another ( such as 0 to 1 or 1 to 0), the output signal would change into 1, otherwise it would maintain 0. Thus, the immediate response to emergency (input change) enables our design to detect signal variation in a short time, which is beneficial for process control because of its time-saving character.

2.Construction of Biological Circuit

Modeling

we construct the model to simulate the behaver of the system.The GFP used in our project will not degradate in short time which makes the GFP fluorescence of experiment result not go down,but in the model we avoid this problem. 1.Construction of Mathematical Model

2.Model Result
2.1Model of the whole system
2.2Change the degradation speed of AHL
2.3parameter sensitivity

Experiment

1.Ligation Strategy and Parts Reservoir
2.Experiment Result