From 2007.igem.org

Projects

Design: Wet Lab

Design: Printer

Design: Software

Testing

Construction: The Wetlab

Protocols

Final Result of E.co Lisa

At the time of the Jamboree the final state of our project is unfortunately unfinished. We were unable to complete our bacterial printer system. However, even without the fully working printer system our team still has many acheivements to present. This section summarizes the final results of our work with E. coLisa.

Contributions To The Registry

Sequencing of Agarase

Sequencing of Logic Circut

Our Contributions To The Registry

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Number	Parts	Plasmid	New part name	Type	Notes
1	agarase	pSB1A2	i737020	Basic Part
2	R0084 . A340620 . S01414 . I13504	pSB1AK3	I737010	Project
3	R0083 . A340620 . S01414 . I13504	pSB1AK3	I737012	Project
4	R0082 . A340620 . S01414 . I13504	pSB1AK3	I737014	Project
5	R0040 . A340620 . I13504	pSB1AC3	S03868	Intermediate	Entered as part I13553 and J26010, not available or experience
6	R0040 . J01010 . I13401	pSB1AC3	S03867	Intermediate	Entered as part I714081, but not available
7	R0040 . J01008 . B0015	pSB1AC3	S03863	Intermediate	Is S03862 . B0015
8	R0040 . J01080 . I13401	pSB1AC3	S03864	Intermediate	Is J01109 . I13401
9	R0040 . J23008 . B0015	pSB1AC3	S03866	Intermediate	Is S03865 . B0015
10	R0040 . J01010 . E0040 . S01414 . B0015	pSB1AK3	S03871	Intermediate	Is S03870 . I0462
11	S01414 . I13504	pSB1AK3	S03869	Intermediate
12	R0040 . A340620 . J23008 . B0015	pSB1AK3	S03872	Intermediate	Is F2620 . J23021
13	R0040 . J06801	pSB2K3	S03743	Intermediate

Our team is pleased to report that we have submitted 13 unique parts to the iGEM registry. These parts include the β-agarase gene, our logic circut and several of the intermediate parts we constructed.

Sequencing of Agarase

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We were able to sucessfully isolate β-agarase gene from the Pseudoalteromonas atlantica genome. Here are the results we obtained from sequencing the isolated β-agarase gene. Click on the image below to see the full sequencing information for β-agarase in pdf format.

The sequencing information obtained confirms that we have indeed isolated the β-agarase gene and it is present in the plasmid.

	This is the Reverse Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid
	This is the Forward Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid

Sequencing of Logic Circut

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There were three seperate logic ciructs prepared. Each differed only in the initial promotorer being used. The first circut used part R0084 a promter that is activated by phosphorylated ompR while the other two R0082 and R0083 are repressed by phosphorylated ompR

Our team synthesized plasmids containing our logic circut using each of these promoters. The links below connect to pdf's showing the result of the sequencing for each of these plasmids. The sequencing information confirmed that our logic circut was present in the plasmids.

|R0082 Logic Circut| |R0083 Logic Circut| |R0084 Logic Circut|