From 2007.igem.org

Beta portion
pcr CA1154F/G00101 on 9145-I716072 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
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Alpha portion
Pcr CA1150F/CA1099R on I716077 ()
Product is ADtemp5
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Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716074 (Best yield)