From 2007.igem.org

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Contents 1 Primary Reagents 1.1 Kits 1.2 Enzymes 1.3 Chemicals 1.4 Other 2 Disposables 2.1 TUBES:

Primary Reagents

Kits

• Miniprep kit
• Gel clean-up system
• Site directed mutagenesis (stratagene)
  • Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
  • Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
  • QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
• Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx

Enzymes

• Melbourne/primary Restriction enzymes
  • NotI,
  • EcoRI,
  • XbaI,
  • HindIII,
  • SpeI,
• Ligase
• DH5a Competent cells
• DNA ladder

Chemicals

• Agar
• Agarose
• Ethidium Bromide
• EDTA
• Glycerol
• Loading dye for DNA gel
• NaCl
• TRIS

Other

• ice
• milliQ water
• Distilled water

Disposables

• Parrafilm
• Tips (10, 200, 1000 ul)
• Gloves (Large , Med, Small)
• Scalpels
• Tube racks
• Freezer boxes

TUBES:

• Falcon: 10ml, 50ml
• microcentrifuge: 1.7ml
• PCR