Paris/July 6

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Preparing growth medium for transduction screening

If the transduction works (i.e. an homologous recombinaison that should delete DapA by inserting an erythromycin resistant cassette), transducted MG1655 Coli should grow in a medium LB+erythro+ DAP but can't grow in a medium LB+erythro without DAP.

  • Preparation of DAP solution from the powder (50mM). See Protocols
  • Making 10 petri dish (LB+tet+citrate+DAP). See Protocols
  • Making 10 petri dish (LB+erythromycin+citrate+DAP). See Protocols

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