Paris/Freezer
From 2007.igem.org
Contents |
PCR products
| PCR products | ||||
|---|---|---|---|---|
| Number | Name | Length | Comments | |
| 1 | Lox71-FtsA-FtsZ-1 | 1537 | ||
| 2 | FtsZ-2 | 1088 | ||
| 3 | DGAT-1 | |||
| 4 | DGAT-2 | |||
| 5 | Lox66-DapAColi | 985 | ||
| 6 | Lox66-DapASubtilis | 979 | ||
| 7 | Lox71-FtsZ | 1260 | EcoR1 site not mutated | |
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | ||
| 1 | pJ23100 | Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| 2 | pJ23107 | Medium constitutive promoter in J61002 (clones 1 + 2) | ||
| 3 | B0015 | Double terminator in pSB1AK3 (clones 1 + 2) | ||
| 3 | I0500 | AraC pBAD in pSB2K3 (clones 1 + 2) | ||
Digestion Products
| Digestion Products | ||||
|---|---|---|---|---|
| Number | Name | Enzyme 1 | Enzyme 2 | Description |
| 1 | pJ23100 | EcoR1 | Spe1 | |
| 2 | pJ23100 | Spe1 | Pst1 | |
| 3 | pJ23107 | EcoR1 | Spe1 | |
| 4 | pJ23107 | Spe1 | Pst1 | |
| 5 | pSB2K3 | EcoR1 | Pst1 | |
| 6 | pSB1AK3 | EcoR1 | Pst1 | |
| 6 | Lox66 DapA E.coli (PCR product) | EcoR1 | Pst1 | |
| 6 | Lox66 DapA E.coli (PCR product) | Xba1 | Pst1 | |
Strains (glycerol stock)
- MG1655
- Acinetobacter ADP1
- w121
- p2C (CFP fluorescent)
- p2Y (YFP fluorescent)
- FtsZTS strains
- MG1655 FtsZ -TS 84
- 121.1
- 121.2
- 121.3
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3
- DH5alpha pKs::DGAT
- pJ23100 Clone1
- pJ23100 Clone2
- BBa_B0015 Clone1
- BBa_B0015 Clone2
