From 2007.igem.org
Study of the pBAD promoter
We've put the AraC-pBAD promoter (BBa_I0500) in the expression vector BBa_J61002 (between EcoRI and SpeI). After overnight culture of E.coli transformed by this plamid, we diluted 1/100 and waited 2 hours. At OD=0.2, we divided the culture in 2 subcultures, one with arabinose (final 0.2%) and one without. 1 hours later, we took pictures with fluorescence microscope.
Digestion reactions
| Digestion Products
|
| Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
|
|
|
|
| D37'.1
| lox71-gfp(tripart) BI
| L12.1 miniprep product (from 30/07 ligation reaction)
| XbaI
| PstI
|
| lox71_RBS-rfp-double terminator extracted as a backward insert
|
|
|
|
| D39'
| BI-insert lox66-DapAE.coli
| lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
| XbaI
| PstI
|
| lox66-dapAcoli extracted for usage as a backward insert
|
|
|
|
| D41
| araC/pBad-J61002 plasmid SP dig.
| L1.2, L1".1 & L1".2 miniprep product
| SpeI
| PstI
|
| J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS
|
|
|
|
Minipreps
| Number
| Name
| Description
|
| MPT2
|
| S16.2
|
| MPT3.1
|
| pTOPO P6.1
|
| MPT3.2
|
| pTOPO P6.7
|
| MPT4.1
|
| pTOPO P8.2
|
| MPT4.2
|
| pTOPO P8.5
|
| MPT4.3
|
| pTOPO P8.7
|
| MPT4.4
|
| pTOPO P8.8
|
| MPT4.5
|
| pTOPO P8.10
|
| MPT5.1
|
| pTOPO P9.1
|
| MPT5.2
|
| pTOPO P9.2
|
| MPT5.3
|
| pTOPO P9.4
|
