From 2007.igem.org

Using Matlab we simulated the behavior of the system when o.a. concentration varies. Then, we plotted the steady state values of PHO80PHO85 (red light) and PHO8 (green light). We started by examining the results using the first couple of input functions, i.e. saturating at 4mM and 12mM. The following pictures show the results of this analysis when K1 varies.

It's clear that k1=0.1 and K1=0.5 doesn't ensure a correct behavior of the system: the repression of PHO8 is too weak when the oleic acid concentration becomes greater than 7.1 mM! We repeated the same analysis for the varying parameters K2 and K3 and we found that a good behavior is given by k2=1 and k3=0.1. This is confirmed be the following pictures:

Using the second couple of input functions, i.e saturating at 4mM and 6mM, we obtain: [INSERT THE PICTURES HERE]