From 2007.igem.org

For a detailed descrition of the lab work please go to the Lab Diary section.

Experiments

we will first start validating our model. we will use pOmpR as both inputs of the system and asses the dynamic behaviour of the system as well as experiments varying the osmolarity of the medium. this way we hope to define the values of our equations, thus taking the leap from an effective model to a model that describes more correctly the system we work on.

then, with this corrected model, we will be able to precisely study the differences between pOmpR and pOmpRm and to calibrate them. This way, we could also use this model to make some good predictions about what parameters we could tune in order to make the system behave the way we want.

once we have calibrated pOmpR and pOmpRm, we will aim to a third variant of this promoter that will have different strenght.