From 2007.igem.org

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Aug 2007: Order ComP and ComA from GENEART

We were counting on [http://partsregistry.org/Part:BBa_J51000 ComP] and [http://partsregistry.org/Part:BBa_J51001 ComA] being available in the 2007 iGEM distribution. As this was not the case, and we had DNA synthesis bases to burn, we ended up ordering them from GENEART. The original sequences, as described in the above links, were optimized for expression in e.coli and all cis-acting sites and assembly restriction sites were removed. We also included biobrick prefix/suffix and vf2 and vr primer binding sites. The two sequences, now labeled ComA' and ComP' took around 3 weeks after confirmation of order to be dispatched. The sequences have been uploaded below:

ComA' sequence (As ordered sequence)

ComP' sequence (As ordered sequence)

Aug 2007: Primer design for PCR stitching

In order to stitch Nt-SopII to ComP at the sites detailed here, these primers were designed. We aimed to have at least 18bp on each half of the stitch, while adhering to the standard primer design guidelines.

-- Oligo 1 --

  Sequence Name: BL_Con1_s
  Sequence: Cagtcggtgcggacatcgttgcttgtaagcaaagcttatac
  Number of Bases: 41

-- Oligo 2 --

  Sequence Name: BL_Con1_as
  Sequence: GTATAAGCTTTGCTTACAAGCAACGATGTCCGCACCGACTG
  Number of Bases: 41

-- Oligo 3 --

  Sequence Name: BL_Con2_s
  Sequence: TcggtgcggacatcgctgCTTGTAAGCAAAGCTTATACCTTTG
  Number of Bases: 43

-- Oligo 4 --

  Sequence Name: BL_Con2_as
  Sequence: CAAAGGTATAAGCTTTGCTTACAAGCAGCGATGTCCGCACCGA
  Number of Bases: 43

-- Oligo 5 --

  Sequence Name: BL_Con3_s
  Sequence: GGTGCGGACATCGCTGGAGGTAAGCAAAGCTTATACCTTTGAG
  Number of Bases: 43

-- Oligo 6 --

  Sequence Name: BL_Con3_as
  Sequence: CTCAAAGGTATAAGCTTTGCTTACCTCCAGCGATGTCCGCACC
  Number of Bases: 43

-- Oligo 7 --

  Sequence Name: BL_Con4_s
  Sequence: GCGGACATCGCTGGAGGACAGCAAAGCTTATACCTTTGAGGT
  Number of Bases: 42

-- Oligo 8 --

  Sequence Name: BL_Con4_as
  Sequence: ACCTCAAAGGTATAAGCTTTGCTGTCCTCCAGCGATGTCCGC
  Number of Bases: 42

-- Oligo 9 --

  Sequence Name: BL_Con5_s
  Sequence: GACATCGCTGGAGGACGCCAAAGCTTATACCTTTGAGGTTACTC
  Number of Bases: 44

-- Oligo 10 --

  Sequence Name: BL_Con5_as
  Sequence: GAGTAACCTCAAAGGTATAAGCTTTGGCGTCCTCCAGCGATGTC
  Number of Bases: 44

-- Oligo 11 --

  Sequence Name: BL_Con6_s
  Sequence: gacgcagagcaggcacaaaagGTGATATTTTTAGATAAGCATGAAGTT
  Number of Bases: 48

-- Oligo 12 --

  Sequence Name: BL_Con6_as
  Sequence: AACTTCATGCTTATCTAAAAATATCACCTTTTGTGCCTGCTCTGCGTC
  Number of Bases: 48

-- Oligo 13 --

  Sequence Name: BL_Con7_s
  Sequence: GGCAGAGGAGATCAACACGGAAGTTGGACCGGACTGGAATT
  Number of Bases: 41

-- Oligo 14 --

  Sequence Name: BL_Con7_as
  Sequence: AATTCCAGTCCGGTCCAACTTCCGTGTTGATCTCCTCTGCC
  Number of Bases: 41

-- Oligo 15 --

  Sequence Name: BL_FP1_s
  Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT
  Number of Bases: 48

-- Oligo 16 --

  Sequence Name: NL26-F
  Sequence: GAATTCGCGGCCGCTTCTAGATAGAAGGATTTACGCGC
  Number of Bases: 38