From 2007.igem.org

Works top　　0.Hybrid promoter　　1.Formulation　　2.Assay1　　3.Simulation　　4.Assay2　　5.Future works

1.Important assay

Hill function fitting

新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.

導出に関してはfor more detail.

Assays for hybrid promoter

These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.

Repression and activation check with LacI and AHL

目的

結果

IPTG assay

目的

結果

Activation check by cell-produced AHL

目的

結果

2.Preliminary assays

We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
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